Fin whale song evolution in the North Atlantic.

Animal songs can change within and between populations as the result of different evolutionary processes. When these processes include cultural transmission, the social learning of information or behaviours from conspecifics, songs can undergo rapid evolutions because cultural novelties can emerge more frequently than genetic mutations. Understanding these song variations over large temporal and spatial scales can provide insights into the patterns, drivers and limits of song evolution that can ultimately inform on the species' capacity to adapt to rapidly changing acoustic environments. Here, we analysed changes in fin whale (Balaenoptera physalus) songs recorded over two decades across the central and eastern North Atlantic Ocean. We document a rapid replacement of song INIs (inter-note intervals) over just four singing seasons, that co-occurred with hybrid songs (with both INIs), and a clear geographic gradient in the occurrence of different song INIs during the transition period. We also found gradual changes in INIs and note frequencies over more than a decade with fin whales adopting song changes. These results provide evidence of vocal learning in fin whales and reveal patterns of song evolution that raise questions on the limits of song variation in this species.

Effects of vessel traffic and ocean noise on gray whale stress hormones.

Human use of marinescapes is rapidly increasing, especially in populated nearshore regions where recreational vessel traffic can be dense. Marine animals can have a physiological response to such elevated human activity that can impact individual health and population dynamics. To understand the physiological impacts of vessel traffic on baleen whales, we investigated the adrenal stress response of gray whales (Eschrichtius robustus) to variable vessel traffic levels through an assessment of fecal glucocorticoid metabolite (fGC) concentrations. This analysis was conducted at the individual level, at multiple temporal scales (1-7 days), and accounted for factors that may confound fGC: sex, age, nutritional status, and reproductive state. Data were collected in Oregon, USA, from June to October of 2016-2018. Results indicate significant correlations between fGC, month, and vessel counts from the day prior to fecal sample collection. Furthermore, we show a significant positive correlation between vessel traffic and underwater ambient noise levels, which indicates that noise produced by vessel traffic may be a causal factor for the increased fGC. This study increases knowledge of gray whale physiological response to vessel traffic and may inform management decisions regarding regulations of vessel traffic activities and thresholds near critical whale habitats.

An open access dataset for developing automated detectors of Antarctic baleen whale sounds and performance evaluation of two commonly used detectors.

Since 2001, hundreds of thousands of hours of underwater acoustic recordings have been made throughout the Southern Ocean south of 60° S. Detailed analysis of the occurrence of marine mammal sounds in these circumpolar recordings could provide novel insights into their ecology, but manual inspection of the entirety of all recordings would be prohibitively time consuming and expensive. Automated signal processing methods have now developed to the point that they can be applied to these data in a cost-effective manner. However training and evaluating the efficacy of these automated signal processing methods still requires a representative annotated library of sounds to identify the true presence and absence of different sound types. This work presents such a library of annotated recordings for the purpose of training and evaluating automated detectors of Antarctic blue and fin whale calls. Creation of the library has focused on the annotation of a representative sample of recordings to ensure that automated algorithms can be developed and tested across a broad range of instruments, locations, environmental conditions, and years. To demonstrate the utility of the library, we characterise the performance of two automated detection algorithms that have been commonly used to detect stereotyped calls of blue and fin whales. The availability of this library will facilitate development of improved detectors for the acoustic presence of Southern Ocean blue and fin whales. It can also be expanded upon to facilitate standardization of subsequent analysis of spatiotemporal trends in call-density of these circumpolar species.

Exploring movement patterns and changing distributions of baleen whales in the western North Atlantic using a decade of passive acoustic data.

Six baleen whale species are found in the temperate western North Atlantic Ocean, with limited information existing on the distribution and movement patterns for most. There is mounting evidence of distributional shifts in many species, including marine mammals, likely because of climate-driven changes in ocean temperature and circulation. Previous acoustic studies examined the occurrence of minke (Balaenoptera acutorostrata) and North Atlantic right whales (NARW; Eubalaena glacialis). This study assesses the acoustic presence of humpback (Megaptera novaeangliae), sei (B. borealis), fin (B. physalus), and blue whales (B. musculus) over a decade, based on daily detections of their vocalizations. Data collected from 2004 to 2014 on 281 bottom-mounted recorders, totaling 35,033 days, were processed using automated detection software and screened for each species' presence. A published study on NARW acoustics revealed significant changes in occurrence patterns between the periods of 2004-2010 and 2011-2014; therefore, these same time periods were examined here. All four species were present from the Southeast United States to Greenland; humpback whales were also present in the Caribbean. All species occurred throughout all regions in the winter, suggesting that baleen whales are widely distributed during these months. Each of the species showed significant changes in acoustic occurrence after 2010. Similar to NARWs, sei whales had higher acoustic occurrence in mid-Atlantic regions after 2010. Fin, blue, and sei whales were more frequently detected in the northern latitudes of the study area after 2010. Despite this general northward shift, all four species were detected less on the Scotian Shelf area after 2010, matching documented shifts in prey availability in this region. A decade of acoustic observations have shown important distributional changes over the range of baleen whales, mirroring known climatic shifts and identifying new habitats that will require further protection from anthropogenic threats like fixed fishing gear, shipping, and noise pollution.

Multi-year occurrence of sei whale calls in North Atlantic polar waters.

In 2009-2014, autonomous hydrophones were deployed on established long-term moorings in the Fram Strait and Greenland Sea to record multi-year, seasonal occurrence of vocalizing cetaceans. Sei whales have rarely been observed north of ∼72°N, yet there was acoustic evidence of sei whale presence in the Fram Strait for several months during all five years of the study. More sei whale calls were recorded at the easternmost moorings in the Fram Strait, likely because of the presence of warm Atlantic water and a strong front concentrating prey in this area. Sei whale vocalizations were not recorded at the Greenland Sea 2009-2010 mooring, either because this area is not part of the northward migratory path of sei whales or because oceanographic conditions were not suitable for foraging. No clear relationship between whale presence and water temperature data collected coincident with acoustic data was observed, but decadal time series of water temperature data collected in the eastern Fram Strait by others exhibit a warming trend, which may make conditions suitable for sei whales. Continued monitoring of the region will be required to determine if the presence of sei whales in these polar waters is ephemeral or a common occurrence.

Two unit analysis of Sri Lankan pygmy blue whale song over a decade.

Sri Lankan pygmy blue whale song consists of three repeated units: (1) low frequency pulsive unit, (2) frequency modulated (FM) upsweep, and (3) long tonal downsweep. The Unit 2 FM unit has up to three visible upsweeps with energy concentrated at approximately 40, 50, and 60 Hz, while the Unit 3 (∼100 Hz) tonal downsweep is the most distinct unit lasting 20-30 s. Spectral characteristics of the Units 2 and 3 song elements, along with ocean sound levels, were analyzed in the Indian Ocean from 2002 to 2013. The peak frequency of the tonal Unit 3 calls decreased from approximately 106.5 to 100.7 Hz over a decade corresponding to a 5.4% decrease. Over the same time period, the frequency content of the Unit 2 upsweeps did not change as dramatically with only a 3.1% change. Ambient sound levels in the vocalization bands did not exhibit equivalent patterns in amplitude trends. Analysis showed no increase in the ambient sound or compensated peak amplitude levels of the tonal downsweeps, eliminating the presence of a Lombard effect. Here it is proposed that each song unit may convey different information and thus may be responding to different selective pressures.

Long-term passive acoustic recordings track the changing distribution of North Atlantic right whales (Eubalaena glacialis) from 2004 to 2014.

Given new distribution patterns of the endangered North Atlantic right whale (NARW; Eubalaena glacialis) population in recent years, an improved understanding of spatio-temporal movements are imperative for the conservation of this species. While so far visual data have provided most information on NARW movements, passive acoustic monitoring (PAM) was used in this study in order to better capture year-round NARW presence. This project used PAM data from 2004 to 2014 collected by 19 organizations throughout the western North Atlantic Ocean. Overall, data from 324 recorders (35,600 days) were processed and analyzed using a classification and detection system. Results highlight almost year-round habitat use of the western North Atlantic Ocean, with a decrease in detections in waters off Cape Hatteras, North Carolina in summer and fall. Data collected post 2010 showed an increased NARW presence in the mid-Atlantic region and a simultaneous decrease in the northern Gulf of Maine. In addition, NARWs were widely distributed across most regions throughout winter months. This study demonstrates that a large-scale analysis of PAM data provides significant value to understanding and tracking shifts in large whale movements over long time scales.

A complex baleen whale call recorded in the Mariana Trench Marine National Monument.

In fall 2014 and spring 2015, passive acoustic data were collected via autonomous gliders east of Guam in an area that included the Mariana Trench Marine National Monument. A short (2-4 s), complex sound was recorded that features a ∼38 Hz moan with both harmonics and amplitude modulation, followed by broad-frequency metallic-sounding sweeps up to 7.5 kHz. This sound was recorded regularly during both fall and spring surveys. Aurally, the sound is quite unusual and most resembles the minke whale "Star Wars" call. It is likely this sound is biological and produced by a baleen whale.

Temporal segregation of the Australian and Antarctic blue whale call types (Balaenoptera musculus spp.).

We examined recordings from a 15-month (May 2009-July 2010) continuous acoustic data set collected from a bottom-mounted passive acoustic recorder at a sample frequency of 6kHz off Portland, Victoria, Australia (38°33'01″S, 141°15'13″E) off southern Australia. Analysis revealed that calls from both subspecies were recorded at this site, and general additive modeling revealed that the number of calls varied significantly across seasons. Antarctic blue whales were detected more frequently from July to October 2009 and June to July 2010, corresponding to the suspected breeding season, while Australian blue whales were recorded more frequently from March to June 2010, coinciding with the feeding season. In both subspecies, the number of calls varied with time of day; Antarctic blue whale calls were more prevalent in the night to early morning, while Australian blue whale calls were detected more often from midday to early evening. Using passive acoustic monitoring, we show that each subspecies adopts different seasonal and daily call patterns which may be related to the ecological strategies of these subspecies. This study demonstrates the importance of passive acoustics in enabling us to understand and monitor subtle differences in the behavior and ecology of cryptic sympatric marine mammals.

Calls reveal population structure of blue whales across the southeast Indian Ocean and the southwest Pacific Ocean.

For effective species management, understanding population structure and distribution is critical. However, quantifying population structure is not always straightforward. Within the Southern Hemisphere, the blue whale (Balaenoptera musculus) complex is extremely diverse but difficult to study. Using automated detector methods, we identified "acoustic populations" of whales producing region-specific call types. We examined blue whale call types in passive acoustic data at sites spanning over 7,370 km across the southeast Indian Ocean and southwest Pacific Ocean (SWPO) from 2009 to 2012. In the absence of genetic resolution, these acoustic populations offer unique information about the blue whale population complex. We found that the Australian continent acts as a geographic boundary, separating Australia and New Zealand blue whale acoustic populations at the junction of the Indian and Pacific Ocean basins. We located blue whales in previously undocumented locations, including the far SWPO, in the Tasman Sea off the east coast of Australia, and along the Lau Basin near Tonga. Our understanding of population dynamics across this broad scale has significant implications to recovery and conservation management for this endangered species, at a regional and global scale.

Seasonal migrations of North Atlantic minke whales: novel insights from large-scale passive acoustic monitoring networks.

BACKGROUND: Little is known about migration patterns and seasonal distribution away from coastal summer feeding habitats of many pelagic baleen whales. Recently, large-scale passive acoustic monitoring networks have become available to explore migration patterns and identify critical habitats of these species. North Atlantic minke whales (Balaenoptera acutorostrata) perform seasonal migrations between high latitude summer feeding and low latitude winter breeding grounds. While the distribution and abundance of the species has been studied across their summer range, data on migration and winter habitat are virtually missing. Acoustic recordings, from 16 different sites from across the North Atlantic, were analyzed to examine the seasonal and geographic variation in minke whale pulse train occurrence, infer information about migration routes and timing, and to identify possible winter habitats. RESULTS: Acoustic detections show that minke whales leave their winter grounds south of 30° N from March through early April. On their southward migration in autumn, minke whales leave waters north of 40° N from mid-October through early November. In the western North Atlantic spring migrants appear to track the warmer waters of the Gulf Stream along the continental shelf, while whales travel farther offshore in autumn. Abundant detections were found off the southeastern US and the Caribbean during winter. Minke whale pulse trains showed evidence of geographic variation, with longer pulse trains recorded south of 40° N. Very few pulse trains were recorded during summer in any of the datasets. CONCLUSION: This study highlights the feasibility of using acoustic monitoring networks to explore migration patterns of pelagic marine mammals. Results confirm the presence of minke whales off the southeastern US and the Caribbean during winter months. The absence of pulse train detections during summer suggests either that minke whales switch their vocal behaviour at this time of year, are absent from available recording sites or that variation in signal structure influenced automated detection. Alternatively, if pulse trains are produced in a reproductive context by males, these data may indicate their absence from the selected recording sites. Evidence of geographic variation in pulse train duration suggests different behavioural functions or use of these calls at different latitudes.

Seasonal presence of cetaceans and ambient noise levels in polar waters of the North Atlantic.

In 2009 two calibrated acoustic recorders were deployed in polar waters of the North Atlantic to study the seasonal occurrence of blue, fin, and sperm whales and to assess current ambient noise levels. Sounds from these cetaceans were recorded at both locations in most months of the year. During the summer months, seismic airguns associated with oil and gas exploration were audible for weeks at a time and dominated low frequency noise levels. Noise levels might further increase in the future as the receding sea ice enables extended human use of the area.

Sounds from airguns and fin whales recorded in the mid-Atlantic Ocean, 1999-2009.

Between 1999 and 2009, autonomous hydrophones were deployed to monitor seismic activity from 16° N to 50° N along the Mid-Atlantic Ridge. These data were examined for airgun sounds produced during offshore surveys for oil and gas deposits, as well as the 20 Hz pulse sounds from fin whales, which may be masked by airgun noise. An automatic detection algorithm was used to identify airgun sound patterns, and fin whale calling levels were summarized via long-term spectral analysis. Both airgun and fin whale sounds were recorded at all sites. Fin whale calling rates were higher at sites north of 32° N, increased during the late summer and fall months at all sites, and peaked during the winter months, a time when airgun noise was often prevalent. Seismic survey vessels were acoustically located off the coasts of three major areas: Newfoundland, northeast Brazil, and Senegal and Mauritania in West Africa. In some cases, airgun sounds were recorded almost 4000 km from the survey vessel in areas that are likely occupied by fin whales, and at some locations airgun sounds were recorded more than 80% days/month for more than 12 consecutive months.

Confirmation of right whales near a nineteenth-century whaling ground east of southern Greenland.

North Atlantic right whales (Eubalaena glacialis) were found in an important nineteenth century whaling area east of southern Greenland, from which they were once thought to have been extirpated. In 2007-2008, a 1-year passive acoustic survey was conducted at five sites in and near the 'Cape Farewell Ground', the former whaling ground. Over 2000 right whale calls were recorded at these sites, primarily during July-November. Most calls were northwest of the historic ground, suggesting a broader range in this region than previously known. Geographical and temporal separation of calls confirms use of this area by multiple animals.

Types, distribution, and seasonal occurrence of sounds attributed to Bryde's whales (Balaenoptera edeni) recorded in the eastern tropical Pacific, 1999-2001.

Vocalizations resembling known Bryde's whale sounds were recorded on autonomous hydrophones at seven sites in the eastern tropical Pacific. Five short (<3 s) low-frequency (<80 Hz) "phrase" types were observed. "Swept alternating tonal" phrases included a 37-Hz tone and often a 25-16-Hz downswept tone, while "nonswept alternating tonal" phrases had a predominant tone at 29 Hz and often additional tones at 16 and 47 Hz. Alternating tonal phrases were found in 79% of the total hours in which phrases were detected, and occurred primarily at the eastern hydrophone sites. "Burst-tonal" phrases included tones that were often preceded by a wideband burst of noise. The "low burst-tonal" phrase contained tones at 19 and 30 Hz, and was detected at five of the hydrophone sites. The "high burst-tonal" phrase included a 42-Hz tone and was observed only on the northwestern hydrophones. A single "harmonic tone" phrase type was observed that included a fundamental tone at 26 Hz and at least two harmonics; this phrase was observed exclusively at the eastern hydrophone stations. This opportunistic survey has shown that acoustics is an effective means of studying this poorly understood, pelagic balaenopterid.

Low-frequency whale and seismic airgun sounds recorded in the mid-Atlantic Ocean.

Beginning in February 1999, an array of six autonomous hydrophones was moored near the Mid-Atlantic Ridge (35 degrees N-15 degrees N, 50 degrees W-33 degrees W). Two years of data were reviewed for whale vocalizations by visually examining spectrograms. Four distinct sounds were detected that are believed to be of biological origin: (1) a two-part low-frequency moan at roughly 18 Hz lasting 25 s which has previously been attributed to blue whales (Balaenoptera musculus); (2) series of short pulses approximately 18 s apart centered at 22 Hz, which are likely produced by fin whales (B. physalus); (3) series of short, pulsive sounds at 30 Hz and above and approximately 1 s apart that resemble sounds attributed to minke whales (B. acutorostrata); and (4) downswept, pulsive sounds above 30 Hz that are likely from baleen whales. Vocalizations were detected most often in the winter, and blue- and fin whale sounds were detected most often on the northern hydrophones. Sounds from seismic airguns were recorded frequently, particularly during summer, from locations over 3000 km from this array. Whales were detected by these hydrophones despite its location in a very remote part of the Atlantic Ocean that has traditionally been difficult to survey.